Website ania_karnkowska University of Warsaw, Faculty of Biology, Institute of Evolutionary Biology

The phenomenon of endosymbiosis – in which one organism lives within another – led to major evolutionary transitions such as the origin of mitochondria and plastids. We currently know that there have been several independent events of endosymbiosis in early eukaryotic evolution, giving rise in some cases to major eukaryotic superclades. However, despite its significance, we still do not understand the mechanism of endosymbiotic integration. Only by examining more recent endosymbioses in various phases of integration can we make progress in our understanding of the host-endosymbiont integration process. In this regard, the endosymbioses of microbial eukaryotes, due to the unicellular nature of the host, provide the most suitable systems for studying the establishment of endosymbiosis. However, the number of such models is very limited and does not allow the reconstruction of the mechanisms of integration. Moreover, recent advances in diversity research suggest that intracellular symbioses are common among microbial eukaryotes, providing a golden opportunity to identify unicellular symbiotic systems in intermediate stages of integration.

To identify the intermediate stages of endosymbiotic integration, SYMBIOSTART will combine screening of diverse freshwater environments for microbial eukaryotes and their endosymbionts with studies on established culturable systems. The goal is to provide a wide range of systems in various stages of integration, which will be further studied to highlight the genomic and ultrastructural features that accompany endosymbiont integration using genomics, transcriptomics and microscopy. Overall, SYMBIOSTART will address the most challenging questions about endosymbiotic events: how integration occurs and what is the relative timing of the steps involved.

Specific Tasks

•      Analysis of high-throughput sequencing data: assembly and annotation of genomes, metagenomes and transcriptomes, amplicon data analysis

•      Isolation and culturing of microbial eukaryotes

•      DNA & RNA isolation from environmental samples and cultures, PCR, preparation of libraries for total genome, transcriptome & amplicon sequencing, Nanopore sequencing

•      Publication of the obtained results in conference talks & papers, active participation in publishing original research articles

Requirements

·       Master’s degree in biology, biotechnology or related field

·       Status of a doctoral student at the Doctoral School of Exact and Natural Sciences of the University of Warsaw at the beginning of the contract

·       Being self-motivated, organized, and highly team-oriented

·       Proficiency in English (oral and written)

·       Background in bioinformatics, especially genomics, transcriptomics and metagenomics.

·      Familiarity with microscopy, molecular biology techniques, and microbiology techniques will be an advantage.

What do we offer?

We offer a scholarship for a PhD student in the National Science Centre project Sonata BIS: Unravelling the establishment of endosymbiosis: quest for intermediate evolutionary stages among microbial eukaryotes (SYMBIOSTART)

·       Starting date: 1 October, 2024

·       Scholarship up to 48 months.

·       Location: Institute of Evolutionary Biology, Faculty of Biology, University of Warsaw

Application deadline: 3 June, 2024

Check more details about the project and application process here

To apply for this job email your details to a.karnkowska@uw.edu.pl

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